Effect of ultrasonically-activated irrigation protocols used for regenerative endodontics on removal of dual species biofilm in a three-dimensionally printed tooth model: in vitro study.

ABSTRACT:

Introduction

Eradication of residual biofilm from root canal dentine is critical for the success of regenerative endodontic procedures (REPs).

The aim of the study

To evaluate the influence of ultrasonically activated irrigants in concentrations used for REPs for removal of dual-species biofilm from three-dimensionally printed tooth models with attached dentine samples.

Methodology

Seventy-two three-dimensionally printed teeth models were fabricated with a standardized slot in the apical third of the root to ensure a precise fit with a human root dentine specimen. Dual-species biofilms (comprising Enterococcus faecalis and Streptococcus mutans) were cultivated in the root canal for a period of three weeks. Models with dentine specimens were randomly assigned into 5 gr

SUBMITTER: Mustafa S 

PROVIDER: S-EPMC11743002 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature