Quantifying intratumoral biomarker heterogeneity in tubo-ovarian high-grade serous carcinoma to optimize clinical translation.

ABSTRACT: Intratumoral heterogeneity (ITH) is spatial, phenotypic, or molecular differences within the same tumor that have important implications for accurate tumor classification and assessment of predictive biomarkers. The Canadian Ovarian Experimental Unified Resource (COEUR) has created a cohort of 437 FFPE tissue specimens from 108 tubo-ovarian high-grade serous carcinoma (HGSC) patients to quantify ITH across the anatomical sites and between primary and recurrence. We quantified the ITH of six clinically used immunohistochemical diagnostic and prognostic biomarkers (WT1, p53, p16, PR, CD8, and Ki67). Markers were stained on tissue microarrays and scored using a continuous or categorical interpretation of staining patterns. Two-way random effect and nested intraclass correlation were used to a

SUBMITTER: Talhouk A 

PROVIDER: S-EPMC11743601 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature