ASB1 engages with ELOB to facilitate SQOR ubiquitination and H₂S homeostasis during spermiogenesis.

ABSTRACT: Male infertility, frequently driven by oxidative stress, impacts half of infertile couples globally. Despite its significance, the precise mechanisms governing this process remain elusive. In this study, we demonstrate that ASB1, the substrate recognition subunit of a ubiquitin ligase, is highly expressed in the mouse testis. Mice lacking the Asb1 gene exhibit severe fertility impairment, characterized by oligoasthenoteratozoospermia. Subsequent investigations unveiled that Asb1 knockout (Asb1-KO) mice encountered excessive oxidative stress and decreased hydrogen sulfide (H₂S) levels in their testes, and severe sperm DNA damage. Notably, the compromised fertility and sperm quality in Asb1-KO mice was significantly ameliorated by administering NaHS, a H₂S donor. Mechan

SUBMITTER: Lv J 

PROVIDER: S-EPMC11743861 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature