Rapid detection of RNase-based self-incompatibility in Lysimachia monelli (Primulaceae).

ABSTRACT:

Premise

Primroses famously employ a system that simultaneously expresses distyly and filters out self-pollen. Other species in the Primulaceae family, including Lysimachia monelli (blue pimpernel), also express self-incompatibility (SI), but involving a system with distinct features and an unknown molecular genetic basis.

Methods

We utilize a candidate-based transcriptome sequencing (RNA-seq) approach, relying on candidate T2/S-RNase Class III and S-linked F-box-motif-containing genes and harnessing the unusual evolutionary and genetic features of SI, to examine whether an RNase-based mechanism underlies SI in L. monelli. We term this approach "SI detection with RNA-seq" (SIDR).

Results

The results of sequencing, crossing, population genetics, and molecular evolutiona

SUBMITTER: Ramanauskas K 

PROVIDER: S-EPMC11744440 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature