Online integration of capillary electrophoresis and dual detector Taylor dispersion analysis via a 3D printed instrument.

ABSTRACT: Hydrodynamic radius (R_H) is a descriptive metric of protein structure with the potential to impact drug development, disease diagnosis, and other important research areas of molecular biology. Common instrumental methods for molecular size characterization are disadvantageous due to high sample consumption, measurements made in non-physiological conditions, and/or inaccurate size determinations. Capillary Taylor dispersion analysis (TDA) is a molecular sizing method that utilizes nL sample volumes and achieves absolute size determination without calibration or comparison to standards. One key drawback of TDA is that it reports the concentration-weighted average R_H, which may be limiting in the analysis of complex sample mixtures. Here, we describe the de

SUBMITTER: Atsar FS 

PROVIDER: S-EPMC11744447 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature