Leveraging Engineered <i>Pseudomonas putida</i> Minicells for Bioconversion of Organic Acids into Short-Chain Methyl Ketones.

ABSTRACT: Methyl ketones, key building blocks widely used in diverse industrial applications, largely depend on oil-derived chemical methods for their production. Here, we investigated biobased production alternatives for short-chain ketones, adapting the solvent-tolerant soil bacterium Pseudomonas putida as a host for ketone biosynthesis either by whole-cell biocatalysis or using engineered minicells, chromosome-free bacterial vesicles. Organic acids (acetate, propanoate and butanoate) were selected as the main carbon substrate to drive the biosynthesis of acetone, butanone and 2-pentanone. Pathway optimization identified efficient enzyme variants from Clostridium acetobutylicum and Escherichia coli, tested with both constitutive and inducible expression of the cognate genes. B

SUBMITTER: Kozaeva E 

PROVIDER: S-EPMC11744930 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature