Fine-Tuning Genetic Circuits via Host Context and RBS Modulation.

ABSTRACT: The choice of organism to host a genetic circuit, the chassis, is often defaulted to model organisms due to their amenability. The chassis-design space has therefore remained underexplored as an engineering variable. In this work, we explored the design space of a genetic toggle switch through variations in nine ribosome binding site compositions and three host contexts, creating 27 circuit variants. Characterization of performance metrics in terms of toggle switch output and host growth dynamics unveils a spectrum of performance profiles from our circuit library. We find that changes in host context cause large shifts in overall performance, while modulating ribosome binding sites leads to more incremental changes. We find that a combined ribosome binding site and host context modulation

SUBMITTER: Chan DTC 

PROVIDER: S-EPMC11744933 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature