Genetically-modified activation strategy facilitates the discovery of sesquiterpene-derived metabolites from Penicillium brasilianum.

ABSTRACT: Genome mining has revealed that Penicillium spp. possess numerous down-regulated or cryptic biosynthetic gene clusters (BGCs). This finding hinted that our investigation of fungal secondary metabolomes is limited. Herein, we report a genetically-modified activation strategy to characterize the spectrum of sesquiterpenoids produced by Penicillium brasilianum CGMCC 3.4402. The cryptic or down-regulated pathways were stimulated by constitutive expression of pathway-specific regulator gene berA responsible for berkeleyacetals biosynthesis from Neosartorya glabra. Chemical analysis of the extracts from the mutant strain Pb-OE:berA enabled the isolation of two new compounds including one bisabolene-type arpenibisabolane C (1), one daucane-type arp

SUBMITTER: He W 

PROVIDER: S-EPMC11745945 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature