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Single-cell spatial transcriptomics reveals distinct patterns of dysregulation in non-neuronal and neuronal cells induced by the Trem2^R47H Alzheimer's risk gene mutation.

ABSTRACT: The R47H missense mutation of the TREM2 gene is a known risk factor for development of Alzheimer's Disease. In this study, we analyze the impact of the Trem2^R47H mutation on specific cell types in multiple cortical and subcortical brain regions in the context of wild-type and 5xFAD mouse background. We profile 19 mouse brain sections consisting of wild-type, Trem2^R47H, 5xFAD and Trem2^R47H; 5xFAD genotypes using MERFISH spatial transcriptomics, a technique that enables subcellular profiling of spatial gene expression. Spatial transcriptomics and neuropathology data are analyzed using our custom pipeline to identify plaque and Trem2^R47H-induced transcriptomic dysregulation. We initially analyze cell type-specific transcriptomic alterations induced

SUBMITTER: Johnston KG

PROVIDER: S-EPMC11746152 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature

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Dataset Information

Single-cell spatial transcriptomics reveals distinct patterns of dysregulation in non-neuronal and neuronal cells induced by the Trem2^R47H Alzheimer's risk gene mutation.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Single-cell spatial transcriptomics reveals distinct patterns of dysregulation in non-neuronal and neuronal cells induced by the Trem2R47H Alzheimer's risk gene mutation.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Single-cell spatial transcriptomics reveals distinct patterns of dysregulation in non-neuronal and neuronal cells induced by the Trem2^R47H Alzheimer's risk gene mutation.