Biomimetic bioreactor for potentiated uricase replacement therapy in hyperuricemia and gout.

ABSTRACT:

Introduction

Uricase replacement therapy is a promising approach for managing hyperuricemia and gout but is hindered by challenges such as short blood circulation time, reduced catalytic activity, and excessive hydrogen peroxide (H₂O₂) production. These limitations necessitate innovative strategies to enhance therapeutic efficacy and safety.

Methods

We designed and synthesized RBC@SeMSN@Uri, a red blood cell-coated biomimetic self-cascade bioreactor, which encapsulates uricase (Uri) and a selenium-based nano-scavenger (SeMSN) within RBC membranes. This design aims to reduce immunogenicity, extend systemic circulation, and maintain enzymatic activity. In vitro assays were conducted to evaluate biocompatibility, anti-inflammatory effects, and oxida

SUBMITTER: Yang B 

PROVIDER: S-EPMC11746906 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature