Single-Cell Insights Into Cellular Response in Abdominal Aortic Occlusion-Induced Hippocampal Injury.

ABSTRACT:

Objective

Ischemia-reperfusion of the abdominal aorta often results in damage to distant organs, such as the heart and brain. This cellular heterogeneity within affected tissues complicates the roles of specific cell subsets in abdominal aorta occlusion model (AAO) injury. However, cell type-specific molecular pathology in the hippocampus after ischemia is poorly understood.

Aims

In this study, we adopted a mouse AAO to investigate the single-cell transcriptome in the hippocampi in AAO mice.

Methods

Male C57BL/6 mice (8 weeks old) were used to create an AAO model, with animals divided into Sham and I/R groups. The I/R group was subjected to 2 h of ischemia followed by 24 h of reperfusion, after which hippocampal tissues were collected for single-cell RNA sequencing an

SUBMITTER: Ren C 

PROVIDER: S-EPMC11746957 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature