Single-cell RNA sequencing of the carotid artery and femoral artery of rats exposed to hindlimb unloading.

ABSTRACT:

Background

Prolonged spaceflight is known to cause vascular deconditioning and remodeling. Tail suspension, a widely used spaceflight analog, is reported to result in vascular remodeling of rats. However, little is known about the cellular atlas of the heterogeneous cells of CA and FA from hindlimb-unloaded rats.

Methods

Firstly, we leveraged scRNA-seq to perform clustering analysis to identify diverse cell populations and sub-clusters within CA and FA from rats subjected to 3 months of hindlimb unloading. The dysregulated genes specific for artery types and cell types in HU group compared to Con were unraveled. Then R package "Cellchat" was used to reveal ligand-receptor cellular communication. At last, the TF network analysis was performed using the SCENIC R package to pre

SUBMITTER: Li C 

PROVIDER: S-EPMC11747068 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature