Impact of input volume on red cell quality in deglycerolized RBCs using a modified ACP-215 protocol.

ABSTRACT:

Background

The ACP 215 automated cell processor is used to glycerolize and deglycerolize red cell concentrates (RCCs). Its primary advantage over the COBE 2991, previously used to cryopreserve RCCs, is that it maintains a closed system enabling extended post-thaw expiry. However, it was observed that post-deglycerolization hematocrits (Hct) of units processed with the LN236 kit are markedly lower than those processed using the COBE 2991. Therefore, we intended to determine whether a modified process using a smaller volume deglycerolization kit (LN235) could increase the final Hct with limited deleterious effects on product characteristics.

Study design and methods

Two proof-of-concept (POC) studies, conducted to determine the feasibility of using the LN235 processing kit for

SUBMITTER: Howell A 

PROVIDER: S-EPMC11747085 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature