Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis.

ABSTRACT: Knowledge about how and where proteins interact provides a pillar for cell biology. Protein proximity-labeling has emerged as an important tool to detect protein interactions. Biotin-related proximity labeling approaches are by far the most commonly used but may have labeling-related drawbacks. Here, we use pupylation-based proximity labeling (PUP-IT) as a tool for protein interaction detection in plants. We show that PUP-IT readily confirmed protein interactions for several known protein complexes across different types of plant hosts and that the approach increased detection of specific interactions as compared to biotin-based proximity labeling systems. To further demonstrate the power of PUP-IT, we used the system to identify protein interactions of the protein complex that underpin ce

SUBMITTER: Zheng S 

PROVIDER: S-EPMC11747095 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature