Effective semi-fed-batch saccharification with high lignocellulose loading using co-culture of <i>Clostridium thermocellum</i> and <i>Thermobrachium celere</i> strain A9.

ABSTRACT: Maximizing saccharification efficiency of lignocellulose and minimizing the production costs associated with enzyme requirements are crucial for sustainable biofuel production. This study presents a novel semi-fed-batch saccharification method that uses a co-culture of Clostridium thermocellum and Thermobrachium celere strain A9 to efficiently break down high solid-loading lignocellulosic biomass without the need for any external enzymes. This method optimizes saccharification efficiency and enhances glucose production from alkaline-treated rice straw, a representative lignocellulosic biomass. Initially, a co-culture of C. thermocellum and T. celere strain A9 was established with a treated rice straw loading of 150 g/l, supplemented with Tween 20, which enhanced

SUBMITTER: Nhim S 

PROVIDER: S-EPMC11747163 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature