Vitamin D protects spermatogonia and Sertoli cells from heat stress damage by inhibiting NLRP3.

ABSTRACT:

Introduction

Cryptorchidism can damage cells in the cryptorchid testes due to elevated local temperatures, potentially impacting the fertility of the child in adulthood. Research indicates that vitamin D enhances sperm quality in adult males. This study aimed to explore whether vitamin D inhibits NLRP3 activation, thus helping to mitigate heat stress damage to testicular spermatogenic and Sertoli cells.

Materials and methods

Five cases of normal testicular tissue adjacent to a tumor (testis removed due to tumorous growth) and five cases of atrophied cryptorchid testicular tissue (testis removed) were analyzed for immunohistochemistry to determine NLRP3 expression in cryptorchid tissue. In Phase I, spermatogonia (GC-1) and Sertoli cells (TM4) were separated into blank and hea

SUBMITTER: Chu H 

PROVIDER: S-EPMC11747411 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature