Rational engineering of <i>Escherichia coli</i> strain for stable and enhanced biosynthesis of pinene.

ABSTRACT: Monoterpene α-pinene exhibits significant potential as an alternative fuel, widely recognized for its affordability and eco-friendly nature. It demonstrates multiple biological activities and has a wide range of applications. However, the limited supply of pinene extracted from plants poses a challenge in meeting the needs of the aviation industry and other sectors. Considering this, the microbial cell factory is the only viable option for achieving sustainable pinene production. This study employed a rational design model to optimize the copy number and integration site for the heterogenous pinene expression pathway in Escherichia coli (E. coli). The integrated strain with the best pinene pathway PG1 was selected. Subsequently, the resulting strain, E. coli HSY

SUBMITTER: Hussain MH 

PROVIDER: S-EPMC11747701 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature