Spatially-resolved characterization of the metabolic and <i>N</i>-glycan alterations in colorectal cancer using matrix-assisted laser desorption/ionization mass spectrometry imaging.

ABSTRACT: Colorectal cancer is the second leading cause of cancer-related deaths worldwide, and its development typically involves complex metabolic reprogramming. By mapping the spatial distributions of metabolites and N-glycans in heterogeneous colorectal cancer tissues, we can elucidate cancer-associated metabolic and N-glycan changes. Herein, we combine mass spectrometry imaging-based metabolomics and N-glycomics to characterize the spatially resolved reprogramming of metabolites and N-glycans in colorectal cancer tissues. The metabolic characteristics of different regions of colorectal cancer were evaluated through the utilization of orthogonal partial least squares discriminant analysis. In combination with metabolic pathway enrichment analysis, significant alterati

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11747861 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature