Novel photocrosslinking chemical probes utilized for high-resolution spatial transcriptomics.

ABSTRACT: The architecture of cells and the tissue they form within multicellular organisms are highly complex and dynamic. Cells optimize their function within tissue microenvironments by expressing specific subsets of RNAs. Advances in cell tagging methods enable spatial understanding of RNA expression when merged with transcriptomics. However, these techniques are currently limited by the spatial resolution of the tagging, the number of RNAs that can be sequenced, and multiplexing to isolate spatially-distinct cells within the same tissue landscape. To address these limitations, we developed CrossSeq, which employs photocrosslinking fluorescent probes and confocal microscopy activation to demarcate user-defined regions of interest on fixed cells for multiplexed spatial transcriptomic analysis. We

SUBMITTER: Spitalny L 

PROVIDER: S-EPMC11748054 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature