Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against <i>Pseudomonas aeruginosa</i>.

ABSTRACT: Pyroptosis is an inflammatory immune response of eukaryotic cells to bacterial lipopolysaccharide (LPS) and other pathological stimuli, leading to the activation of the gasdermin D (GSDMD) and secretion of pore-forming domain GSDMD_Nterm, facilitating the release of cytokines. Additionally, GSDMD_Nterm exhibits antibacterial properties through interactions with bacterial outer membranes (OM). We explored alternative antimicrobial strategy to determine whether inducing natural pyroptosis via GSDMD activation by P. aeruginosa LPS could enhance the effectiveness of recombinant phage endopeptidase KP27 (peptidoglycan-degrading enzyme) against P. aeruginosa, enabling penetration through OM and bacterial killing synergistically. Our findings demonstrated that re

SUBMITTER: Kuc-Ciepluch D 

PROVIDER: S-EPMC11748493 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature