Revealing systematic changes in the transcriptome during the transition from exponential growth to stationary phase.

ABSTRACT: The composition of bacterial transcriptomes is determined by the transcriptional regulatory network (TRN). The TRN regulates the transition from one physiological state to another. Here, we use independent component analysis to monitor the composition of the transcriptome during the transition from the exponential growth phase to the stationary phase. With Escherichia coli K-12 MG1655 as a model strain, we trigger the transition using carbon, nitrogen, and sulfur starvation. We find that (i) the transition to the stationary phase accompanies common transcriptome changes, including increased stringent responses and reduced production of cellular building blocks and energy regardless of the limiting element; (ii) condition-specific changes are strongly associated with transcriptional

SUBMITTER: Lim HG 

PROVIDER: S-EPMC11748552 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature