Hypoxia impairs decitabine-induced expression of HLA-DR in acute myeloid leukaemia cell lines.

ABSTRACT:

Background

Hypomethylating agents (HMA), such as azacytidine (AZA) and decitabine (DAC), are epigenetic therapies used to treat some patients with acute myeloid leukaemia (AML) and myelodysplastic syndrome. HMAs act in a replication-dependent manner to remove DNA methylation from the genome. However, AML cells targeted by HMA therapy are often quiescent within the bone marrow, where oxygen levels are low. In this study, we investigate the effects of hypoxia on HMA responses in AML cells.

Results

AML cell lines (MOLM-13, MV-4-11, HL-60) were treated with DAC (100 nM) or AZA (500-2000 nM) in normoxic (21% O₂) and hypoxic (1% O₂) conditions. Hypoxia significantly reduced AML cell growth across all cell lines, with no additional effects observed upon HMA tr

SUBMITTER: Humphries S 

PROVIDER: S-EPMC11748578 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature