Compositional data analysis enables statistical rigor in comparative glycomics.

ABSTRACT: Comparative glycomics data are compositional data, where measured glycans are parts of a whole, indicated by relative abundances. Applying traditional statistical analyses to these data often results in misleading conclusions, such as spurious "decreases" of glycans when other structures increase in abundance, or high false-positive rates for differential abundance. Our work introduces a compositional data analysis framework, tailored to comparative glycomics, to account for these data dependencies. We employ center log-ratio and additive log-ratio transformations, augmented with a scale uncertainty/information model, to introduce a statistically robust and sensitive data analysis pipeline. Applied to comparative glycomics datasets, including known glycan concentrations in defined mixtures

SUBMITTER: Bennett AR 

PROVIDER: S-EPMC11748655 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature