Efficient regeneration protocol for producing true-to-type oil palm (<i>Elaeis guineensis</i> (jacq.) through somatic embryogenesis from immature male inflorescence.

ABSTRACT: Producing homogeneous planting material in oil palm poses a significant challenge, which can be addressed through somatic embryogenesis. This study successfully achieved somatic embryogenesis using immature male inflorescence from Tenera hybrid. Modified Eeuwens medium yielded better results than the Murashige and Skoog (MS) and CHU (N6) media when supplemented with 2,4-D, picloram and α-naphthaleneacetic acid (NAA). The Eeuwens (Y3) medium supplemented with picloram led to an increased callus percentage, ranging from 25 % to 63.7 %. The highest rate of somatic embryogenesis was observed with a combination of 2,4-D (16 mg/l), picloram (24 mg/l), and 2,4,5-trichlorophenoxyacetic acid (2,4,5 -T) (2 mg/l), resulting in an average embryogenesis percentage between 2.54 % and 7.24 %. The average

SUBMITTER: Babu BK 

PROVIDER: S-EPMC11748703 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature