Utilization of primary tumor samples for cancer neoantigen discovery.

ABSTRACT:

Background

The use of tumor-infiltrating T lymphocytes (TIL) that recognize cancer neoantigens has led to lasting remissions in metastatic melanoma and certain cases of metastatic epithelial cancer. For the treatment of the latter, selecting cells for therapy typically involves laborious screening of TIL for recognition of autologous tumor-specific mutations, detected through next-generation sequencing of freshly resected metastatic tumors. Our study explored the feasibility of using archived formalin-fixed, paraffin-embedded (FFPE) primary tumor samples for cancer neoantigen discovery, to potentially expedite this process and reduce the need for resections normally required for tumor sequencing.

Method

Whole-exome sequencing was conducted on matched primary and metastatic c

SUBMITTER: Leko V 

PROVIDER: S-EPMC11748769 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature