Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha.

ABSTRACT:

Background

Ogataea polymorpha, a non-conventional methylotrophic yeast, has demonstrated significant potential for heterologous protein expression and the production of high-value chemicals and biopharmaceuticals. However, the lack of precise and efficient genome editing tools severely hinders the construction of cell factories. Although the CARISP-Cas9 system has been established in Ogataea polymorpha, the gene editing efficiency, especially for multiple genes edition, needs to be further improved.

Results

In this study, we developed an efficient CRISPR-Cpf1-mediated genome editing system in O. polymorpha that exhibited high editing efficiency for single gene (98.1 ± 1.7%), duplex genes (93.9 ± 2.4%), and triplex genes (94.0 ± 6.0%). Additionally, by knocking out non-homolo

SUBMITTER: Hou S 

PROVIDER: S-EPMC11748851 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature