Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease.

ABSTRACT:

Background and objective

Mitochondria are crucial to the function of renal tubular cells, and their dynamic perturbation in many aspects is an important mechanism of diabetic kidney disease (DKD). Single-nucleus RNA sequencing (snRNA-seq) technology is a high-throughput sequencing analysis technique for RNA at the level of a single cell nucleus. Here, our DKD mouse kidney single-cell RNA sequencing conveys a more comprehensive mitochondrial profile, which helps us further understand the therapeutic response of this unique organelle family to drugs.

Methods

After high fat diet (HFD), mice were intraperitoneally injected with streptozotocin (STZ) to induce DKD, and then divided into three subsets: CON (healthy) subset, DKD (vehicle) subset, and LST (losartan; 25 mg/kg/day) sub

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC11748887 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature