A novel ready-to-use loop-mediated isothermal amplification (LAMP) method for detection of Burkholderia mallei and B. pseudomallei.

ABSTRACT:

Background

Glanders and melioidosis are contagious zoonotic diseases caused by Burkholderia mallei and B. pseudomallei, respectively. Bacterial isolation and polymerase chain reaction (PCR) have been used to detect these bacteria in animals suspected of infection; however, both methods require skilled experimental techniques and expensive equipment. These obstacles make it difficult to diagnose B. mallei and B. pseudomallei infections in areas where reagents and equipment are difficult to procure. To solve this problem, we developed an easy and ready-to-use dried-format diagnostic tool based on loop-mediated isothermal amplification (LAMP) method.

Results

The primer set targeting the internal transcribed spacer (ITS) region detected 10 genomic copies of B. mallei DNA and B.

SUBMITTER: Nakase M 

PROVIDER: S-EPMC11749068 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature