Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors.

ABSTRACT:

Background

Glucose deprivation inhibits T-cell metabolism and function. Glucose levels are low in the tumor microenvironment of solid tumors and insufficient glucose uptake limits the antitumor response of T cells. Furthermore, glucose restriction can contribute to the failure of chimeric antigen receptor T (CAR-T) cell therapy for solid tumors. However, the impact of glucose restriction remains unknown in CAR-T cell therapy.

Methods

Glucose transporters were detected and overexpressed in CAR-T cells. The impacts of glucose restriction on CAR-T cells were checked in vitro and in vivo.

Results

Glucose restriction significantly decreased CAR-T cell activation, effector function, and expansion. CAR-T cells expressed high levels of the glucose transporter Glut1, which has

SUBMITTER: Hu W 

PROVIDER: S-EPMC11749199 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature