Specific and sensitive detection of bovine coronavirus using CRISPR-Cas13a combined with RT-RAA technology.

ABSTRACT:

Introduction

Bovine coronavirus (BCoV) is an important pathogen of enteric and respiratory disease in cattle, resulting in huge economic losses to the beef and dairy industries worldwide. A specific and sensitive detection assay for BCoV is critical to the early-stage disease prevention and control.

Methods

We established a specific, sensitive, and stable assay for BCoV nucleic acid detection based on CRISPR/Cas13a combined with reverse transcription recombinase-aided amplification (RT-RAA) technology. The specific primers for RT-RAA and CRISPR RNA (crRNA) were designed in the conserved region of the BCoV nucleocapsid (N) gene.

Results

The detection limit of the RT-RAA CRISPR/Cas13a assays for BCoV detection was 1.72 copies/μl, and there were no cross-reactions with t

SUBMITTER: Liang Z 

PROVIDER: S-EPMC11749252 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature