Immunogenicity and vaccine efficacy of <i>Actinobacillus pleuropneumoniae</i>-derived extracellular vesicles as a novel vaccine candidate.

ABSTRACT: Actinobacillus pleuropneumoniae (APP) is a significant pathogen in the swine industry, leading to substantial economic losses and highlighting the need for effective vaccines. This study evaluates the potential of APP-derived extracellular vesicles (APP-EVs) as a vaccine candidate compared to the commercial Coglapix vaccine. APP-EVs, isolated using tangential flow filtration (TFF) and cushioned ultracentrifugation, exhibited an average size of 105 nm and a zeta potential of -17.4 mV. These EVs demonstrated stability under external stressors, such as pH changes and enzymatic exposure and were found to contain 86 major metabolites. Additionally, APP-EVs induced dendritic cell (DC) maturation in a Toll-like receptor 4 (TLR4)-dependent manner without cytotoxicity. APP-EVs predominantly

SUBMITTER: Hyun Park S 

PROVIDER: S-EPMC11749362 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature