A CRISPR/Cas9-based kinome screen identifies ErbB signaling as a new regulator of human naive pluripotency and totipotency.

ABSTRACT: Regulation of totipotency and naïve pluripotency is crucial for early human embryo development. However, the mechanisms of naïve pluripotency and totipotency regulation in humans, especially the signaling pathways involved in these processes, remain largely unknown. Here, using the conversion of human extended pluripotent stem cells (hEPSCs) to naïve pluripotent stem cells as a model, we performed a CRISPR/Cas9-based kinome knockout screen to analyze the effect of disrupting 763 kinases in regulating human naïve pluripotency. Further validation using small molecules revealed that the inhibition of ErbB family kinases promoted the transition of hEPSCs to human naïve pluripotent stem cells. More importantly, chemical inhibition of the ErbB family also promoted induction of totipotent signatu

SUBMITTER: Li J 

PROVIDER: S-EPMC11749542 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature