Modeling maternal cholesterol exposure reveals a reduction of neural progenitor proliferation using human cerebral organoids.

ABSTRACT: Maternal obesity raises the risk of high-cholesterol exposure for their offspring. Studies in cohorts and animal models report that maternal obesity could increase the risk of neurodevelopmental disorders in offspring including intellectual disabilities and autism spectrum disorders (ASDs). However, whether exposure to high cholesterol is responsible for brain developmental defects, as well as its underlying mechanism, is still unclear. Here, we constructed a cholesterol exposure model utilizing human pluripotent stem cell (hPSC)-derived cerebral organoids by exogenously adding cholesterol into the culture system. We observed enlargement of endosomes, decreased neural progenitor proliferation, and premature neural differentiation in brain organoids with the treatment of cholesterol. Moreov

SUBMITTER: Fan P 

PROVIDER: S-EPMC11749704 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature