Deciphering the dynamic single-cell transcriptional landscape in the ocular surface ectoderm differentiation system.

ABSTRACT: The ocular surface ectoderm (OSE) is essential for the development of the ocular surface, yet the molecular mechanisms driving its differentiation are not fully understood. In this study, we used single-cell transcriptomic analysis to explore the dynamic cellular trajectories and regulatory networks during the in vitro differentiation of embryonic stem cells (ESCs) into the OSE lineage. We identified nine distinct cell subpopulations undergoing differentiation along three main developmental branches: neural crest, neuroectodermal, and surface ectodermal lineages. Key marker gene expression, transcription factor activity, and signaling pathway insights revealed stepwise transitions from undifferentiated ESCs to fate-specified cell types, including a PAX6 + TP63 + population indicativ

SUBMITTER: Zhang C 

PROVIDER: S-EPMC11749776 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature