Deciphering the complex molecular architecture of the genetically modified soybean FG72 through paired-end whole genome sequencing.

ABSTRACT: The clear molecular characterization of genetically modified (GM) plants and animals is a prerequisite for obtaining regulatory approval and safety certification for commercial cultivation. This characterization includes the identification of the transferred DNA (T-DNA) insertion site, its flanking sequences, the copy number of inserted genes, and the detection of any unintended genomic alterations accompanying the transformation process. In this study, we performed a comprehensive molecular characterization of the well-known GM soybean event FG72 using paired-end whole-genome sequencing (PE-WGS). We examined the T-DNA insertion site, flanking sequences, the entire structure and copy number of the T-DNA integration, the presence of plasmid backbone sequences, and genome-wide structural var

SUBMITTER: Wang F 

PROVIDER: S-EPMC11750281 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature