Wilms tumor primary cultures capture phenotypic heterogeneity and facilitate preclinical screening.

ABSTRACT: Wilms tumors (WT) are characterized by variable contributions of blastemal, epithelial and stromal elements, reflecting their diverse cellular origins and genetic drivers. In vitro models remain rare, despite a growing need to better characterize tumor biology and evaluate new treatments. Using three approaches, we have now established a large collection of long-term cultures that represent this diversity. Adherent WT cultures are predominated by stromal cells, 3D spheroids model blastema, and patient-derived organoid cultures of both tumor and healthy kidney tissue result in the preferential growth of epithelial cells. Adherent, spheroid and organoid cultures are also clearly distinguishable by their transcriptome. Preclinical drug screening experiments revealed sensitivity to a range of

SUBMITTER: Gotz L 

PROVIDER: S-EPMC11750297 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature