A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production.

ABSTRACT: Developing a robust and safe carrier for enzyme immobilization is crucial for their application in the food processing industry. In this study, a novel crosslinked chitosan microspheres (CSMs) were prepared using glutaraldehyde (GA) as the crosslinking agent, using a newly developed emulsification-neutralization combined method. Nuclease P1 (NP1) was immobilized onto these microspheres, the maximum activity of NP1@CSMs-GA reach to 53,859.4 U/g. The activity recovery yield reach to 75 %. Compared to the free NP1, the stability of NP1@CSMs-GA was significantly enhanced. Its v_max and K_m is 895.71 mg/(g·min) and 77.27 mg/mL respectively. This NP1@CSMs-GA was utilized in production of nucleotides through hydrolysis of RNA. In BSTR, NP1@CSMs-GA retained more than 75.1 % ini

SUBMITTER: Yin XY 

PROVIDER: S-EPMC11750442 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature