Small molecule-based regulation of gene expression in human astrocytes switching on and off the G-quadruplex control systems.

ABSTRACT: A great deal of attention is being paid to strategies seeking to uncover the biology of the four-stranded nucleic acid structure G-quadruplex (G4) via their stabilization in cells with G4-specific ligands. The conventional definition of chemical biology implies that a complete assessment of G4 biology can only be achieved by implementing a complementary approach involving the destabilization of cellular G4s by ad hoc molecular effectors. We report here on an unprecedented comparison of the cellular consequences of G4 chemical stabilization by pyridostatin (PDS) and destabilization by phenylpyrrolocytosine (PhpC) at both transcriptome- and proteome-wide scales in patient-derived primary human astrocytes. Our results show that the stabilization of G4s by PDS triggers the dysregulation of man

SUBMITTER: M J VK 

PROVIDER: S-EPMC11750478 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature