Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy.

ABSTRACT:

Objective

To explore the differential gene expression in peripheral blood immune cells of individuals with type 2 diabetes mellitus (DM), comparing those with and without non-proliferative diabetic retinopathy (NPDR).

Methods

From a pool of 126 potential participants, 60 were selected for detailed analysis. This group included 12 healthy donors (HDs), 22 individuals with DM, and 26 with NPDR. We analyzed peripheral blood mononuclear cells (PBMCs) using RNA sequencing and quantitative PCR (qPCR) to pinpoint differentially expressed genes (DEGs). Western blot and flow cytometry were also employed to evaluate the protein expression of specific genes.

Results

In patients with NPDR compared to those with DM alone, MerTK-a gene implicated in inherited retinal dystrophies du

SUBMITTER: Bu S 

PROVIDER: S-EPMC11750652 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature