Filamentous cyanobacteria growth assessment using fluorinated ethylene propylene microcapillaries.

ABSTRACT:

Abstract

Filamentous cyanobacteria originate toxic harmful algal blooms (HABs) in aquatic ecosystems, severely impacting freshwater ecosystems and life. Despite being natural bloomers, these microorganisms are challenging to handle in vitro, due to the formation of aggregates with entangled filaments. Consequently, their precise growth dynamics, although vital to timely predict HABs, remains inaccessible. Here, we precisely assessed growth of the HAB forming cyanobacteria Oscillatoria nigroviridis, by cultivating filament suspensions in transparent, gas permeable, and portable fluoropolymer microcapillary strips. Direct optical observation of O. nigroviridis growth revealed shorter filaments comprising less than 50 cells grew at a slower rate, dN/dt = 0.

SUBMITTER: Silva DMS 

PROVIDER: S-EPMC11750922 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature