Proteome analysis of a recombinant Bacillus megaterium strain during heterologous production of a glucosyltransferase.

ABSTRACT: A recombinant B. megaterium strain was used for the heterologous production of a glucosyltransferase (dextransucrase). To better understand the physiological and metabolic responses of the host cell to cultivation and induction conditions, proteomic analysis was carried out by combined use of two-dimensional gel electrophoresis and mass spectrometry (2-DE/MS) for protein separation and identification. 2-DE method was optimized for the separation of intracellular proteins. Since the genome of B. megaterium is not yet available, peptide sequencing using peptide fragment information obtained from nanoelectrospray ionization quadrupole-time-of-flight tandem mass spectrometry (ESI-QqTOF MS/MS) was applied for protein identification. 167 protein spots were identified as 149 individual proteins,

SUBMITTER: Wang W 

PROVIDER: S-EPMC1175100 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature