The transcriptional regulation effects of histidine, isoleucine and glutamate on free exopolysaccharide biosynthesis of <i>Streptococcus thermophilus</i> 937.

ABSTRACT:

Introduction

The free exopolysaccharide (f-EPS) produced by Streptococcus thermophilus is a natural texture modifier and has a variety of prebiotic activities. Our previous studies showed f-EPS production from S. thermophilus 937 was increased 2-fold in the presence of 15 mM of glutamate, isoleucine, and histidine in the chemically defined medium.

Methods

In this study, we used transcriptomics and qPCR to further explore the specific mechanism of the enhanced effect of 3 amino acids on the f-EPS biosynthesis of S. thermophilus 937.

Results

The mRNA-seq analysis and targeted pathway analysis indicated that genes associated with histidine/valine/leucine/ isoleucine/phenylalanine/tyrosine/tryptophan synthesis, galactose metabolism, purine metabolism

SUBMITTER: Wa Y 

PROVIDER: S-EPMC11751036 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature