Hyperpolarized Nano-NMR Platform for Quantification of Mass Limited Samples.

ABSTRACT: Metabolic flux analysis of live cells using NMR enables the study of cancer metabolism and response to treatment. However, conventional NMR platforms require often prohibitively high numbers of cells to achieve significant resolution. In this work, we present a double ¹H/¹³C resonance NMR probe consisting of a solenoid coil with a less than 100 nL sensitive region. In-solution robustness is demonstrated through measurement of decaying hyperpolarized signals. A suspension of live cells and hyperpolarized (HP) [1-¹³C]pyruvate is loaded in the coil, and dynamic changes in pyruvate and lactate concentrations by fractions of femtomoles are detected from just 2000 live cells at a time, in seconds. Through an integrated microfluidic channel, the probe is used as h

SUBMITTER: Pigliapochi R 

PROVIDER: S-EPMC11751133 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature