Rapid and sensitive detection of <i>Haemophilus influenzae</i> using multiple cross displacement amplification combined with CRISPR-Cas12a-based biosensing system.

ABSTRACT: Haemophilus influenzae (H. influenzae, Hi) is an opportunistic bacterium that colonizes the upper respiratory tract of humans and frequently causes meningitis, pneumonia, sepsis, and other severe infections in children. Early and accurate detection of H. influenzae is essential for effective diagnosis and treatment. In this study, we established a novel diagnostic method by integrating the CRISPR-Cas12a detection platform with multiple cross-displacement amplification (MCDA), termed the Hi-MCDA-CRISPR assay. This method offers an efficient and highly precise diagnostic tool for the identification of H. influenzae. In the Hi-MCDA-CRISPR system, the outer membrane protein (OMP) P6 of H. influenzae was pre-amplified using the MCDA assay. The CRISPR-Ca

SUBMITTER: Jia X 

PROVIDER: S-EPMC11751528 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature