Single-Molecule Observation of Competitive Protein-Protein Interactions Utilizing a Nanopore.

ABSTRACT: Two or more protein ligands may compete against each other to interact transiently with a protein receptor. While this is a ubiquitous phenomenon in cell signaling, existing technologies cannot identify its kinetic complexity because specific subpopulations of binding events of different ligands are hidden in the averaging process in an ensemble. In addition, the limited time resolution of prevailing methods makes detecting and discriminating binding events among diverse interacting partners challenging. Here, we utilize a genetically encoded nanopore sensor to disentangle competitive protein-protein interactions (PPIs) in a one-on-one and label-free fashion. Our measurements involve binary mixtures of protein ligands of varying binding affinity against the same receptor, which was externa

SUBMITTER: Sun J 

PROVIDER: S-EPMC11752528 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature