Development of a Komagataella phaffii cell factory for sustainable production of ( +)-valencene.

ABSTRACT:

Background

Sesquiterpene ( +)-valencene is a characteristic aroma component from sweet orange fruit, which has a variety of biological activities and is widely used in industrial manufacturing of food, beverage and cosmetics industries. However, at present, the content in plant sources is low, and its yield and quality would be influenced by weather and land, which limit the supply of ( +)-valencene. The rapid development of synthetic biology has accelerated the construction of microbial cell factories and provided an effective alternative method for the production of natural products.

Results

In this study, we first introduced the ( +)-valencene synthase into Komagataella phaffii by CRISPR/Cas9 system, and successfully constructed a ( +)-valencene producer with the initial

SUBMITTER: Cheng J 

PROVIDER: S-EPMC11752624 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature