Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma.

ABSTRACT:

Background

Uveal melanoma (UM) is the most common intraocular tumor in adults, arises either de novo from normal choroidal melanocytes (NCMs) or from pre-existing nevi that stem from NCMs and are thought to harbor UM-initiating mutations, most commonly in GNAQ or GNA11. However, there are no commercially available NCM cell lines, nor is there a detailed protocol for developing an oncogene-mutated CM line (MutCM) to study UM development. This study aimed to establish and characterize premalignant CM models from human donor eyes to recapitulate the cell populations at the origin of UM.

Results

Given the precious value of human donor eyes for studying multiple ocular cell types, we validated a co-isolation protocol of both human NCMs and retinal pigment epithelial (RPE) cells f

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11752652 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature