Shewanella oneidensis and Methanosarcina barkerii augmentation and conductive material effects on long-term anaerobic digestion performance.

ABSTRACT: This study explores the use of conductive material in scaling up anaerobic digestion for enhanced biogas production. Focusing on Direct Interspecies Electron Transfer (DIET), the research employs a syntrophic DIET-able consortium formed by Shewanella oneidensis and Methanosarcina barkerii in 3.8-L experiments utilizing reticulated vitreous carbon (RVC) as conductive material. In short-term tests with acetate the syntrophic co-culture with RVC resulted in 86% higher maximum velocity of methane production, while in long term with real feed 13% increased rate was observed: the addition of 1.77 (S/m)*m² RVC resulted in a faster methane production of 2.39 mL/gVS*h compared to 2.08 mL/gVS*h of the reference. The experimental conditions of syntrophic inoculum and RVC as conductive mate

SUBMITTER: Perego C 

PROVIDER: S-EPMC11753057 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature