Murine model of cross-IgE sensitization and cross-anaphylactic reactions among multiple group food allergens.

ABSTRACT:

Rationale

Approximately 32 million people in the United States suffer from food allergies. Some food groups, such as legumes - peanuts, tree nuts, fish, and shellfish, have a high risk of cross-reactivity. However, the murine model of multiple food group cross-reactivity is limited.

Objective

We sought to develop a murine model that can be used to investigate novel therapeutics for the treatment of multiple food allergies.

Methods

C3H/HeJ mice were sensitized intraperitoneally (i.p.) once a week for three weeks with a mixture of 500µg of protein from peanut, cashew, walnut, shrimp, cod, and 2 mg Alum. The control group consisted of naïve mice. IgE levels against the sensitized allergens and their cross-reactive allergens were measured by ELISA at baseline and 3 weeks

SUBMITTER: Musa I 

PROVIDER: S-EPMC11753245 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature