A Detailed View on the (Re)isomerization Dynamics in Microbial Rhodopsins Using Complementary Near-UV and IR Readouts.

ABSTRACT: Isomerization is a key process in many (bio)chemical systems. In microbial rhodopsins, the photoinduced isomerization of the all-trans retinal to the 13-cis isomer initiates a cascade of structural changes of the protein. The interplay between these changes and the thermal relaxation of the isomerized retinal is one of the crucial determinants for rhodopsin functionality. It is therefore important to probe this dynamic interplay with chromophore specific markers that combine gapless temporal observation with spectral sensitivity. Here we utilize the near-UV and mid-IR fingerprint region in the framework of a systematic (time-resolved) spectroscopic study on H⁺- (HsBR, (G)PR), Na⁺- (KR2, ErNaR) and Cl^--(NmHR) pumps. We demonstrate that the near-UV region is

SUBMITTER: Asido M 

PROVIDER: S-EPMC11753611 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature